boiling antigen unmasking solution Search Results


tris  (Vector Laboratories)
96
Vector Laboratories tris
Tris, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antigen unmasking solution  (Vector Laboratories)
96
Vector Laboratories antigen unmasking solution
Antigen Unmasking Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectastain abc kit  (Vector Laboratories)
96
Vector Laboratories vectastain abc kit
Vectastain Abc Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tintoretriever pressure chamber  (Bio SB Inc)
90
Bio SB Inc tintoretriever pressure chamber
Tintoretriever Pressure Chamber, supplied by Bio SB Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3  (Bio-Rad)
96
Bio-Rad cd3
Figure 3. b-Arrestin 2 deficiency affects the challenge phase of contact hypersensitivity responses. (a) Representative <t>CD3-stained</t> sections of inflamed ear tissue harvested from Arrb2 / and wild-type (WT) mice 48hours after the second challenge with DNFB (original magnification 200, bar¼ 100 mm). (b) Mean numbers of <t>CD3</t> þ cells per high-power field (HPF) (±SEM, n ¼ 5 per group). (c) Mean mRNA expression levels of Ifng and Tnfa relative to ubiquitin determined by quantitative real-time reverse-transcriptase–PCR (2 DCT ±SEM, n ¼ 5 per group). (d) Experimental protocol for adoptive lymphocyte (LC) transfer experiments. (e) Mean ear swelling 48hours after the second challenge with DNFB (±SEM, n ¼ 5 mice per group). Experiments were repeated two times with similar results. **Po0.01; ***Po0.001.
Cd3, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eif4e  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology eif4e
Male breast cancer (MBC) has a full range of expression patterns for <t>eIF4E,</t> 4E-BP1, 4E-BP2 and p4E-BP1. Tissue microarrays containing multiple tumour cores from 337 MBCs were stained as indicated using immunohistochemistry. Cytoplasmic and nuclear expressions in tumour cells were assessed as 0 (negative) or 2–7 (positive, increasing intensity/proportion of positive cells). Representative positive staining is shown at the top of the panel for each antigen. Images shown were scored for cytoplasmic (c) and nuclear (n) expression as follows: eIF4E – c 7, n 6; 4E-BP1 – c 4, n 5; 4E-BP2 – c 5, n 0; p4E-BP1 – c 6, n 3. Frequency distributions of cytoplasmic (black) or nuclear (grey) expression across the cohort are shown below. Mean scores for each case were determined and are represented rounded to the nearest whole number.
Eif4e, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris-based antigen unmasking solution  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH tris-based antigen unmasking solution
Male breast cancer (MBC) has a full range of expression patterns for <t>eIF4E,</t> 4E-BP1, 4E-BP2 and p4E-BP1. Tissue microarrays containing multiple tumour cores from 337 MBCs were stained as indicated using immunohistochemistry. Cytoplasmic and nuclear expressions in tumour cells were assessed as 0 (negative) or 2–7 (positive, increasing intensity/proportion of positive cells). Representative positive staining is shown at the top of the panel for each antigen. Images shown were scored for cytoplasmic (c) and nuclear (n) expression as follows: eIF4E – c 7, n 6; 4E-BP1 – c 4, n 5; 4E-BP2 – c 5, n 0; p4E-BP1 – c 6, n 3. Frequency distributions of cytoplasmic (black) or nuclear (grey) expression across the cohort are shown below. Mean scores for each case were determined and are represented rounded to the nearest whole number.
Tris Based Antigen Unmasking Solution, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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citrate buffer  (Carl Roth GmbH)
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Carl Roth GmbH citrate buffer
Male breast cancer (MBC) has a full range of expression patterns for <t>eIF4E,</t> 4E-BP1, 4E-BP2 and p4E-BP1. Tissue microarrays containing multiple tumour cores from 337 MBCs were stained as indicated using immunohistochemistry. Cytoplasmic and nuclear expressions in tumour cells were assessed as 0 (negative) or 2–7 (positive, increasing intensity/proportion of positive cells). Representative positive staining is shown at the top of the panel for each antigen. Images shown were scored for cytoplasmic (c) and nuclear (n) expression as follows: eIF4E – c 7, n 6; 4E-BP1 – c 4, n 5; 4E-BP2 – c 5, n 0; p4E-BP1 – c 6, n 3. Frequency distributions of cytoplasmic (black) or nuclear (grey) expression across the cohort are shown below. Mean scores for each case were determined and are represented rounded to the nearest whole number.
Citrate Buffer, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3. b-Arrestin 2 deficiency affects the challenge phase of contact hypersensitivity responses. (a) Representative CD3-stained sections of inflamed ear tissue harvested from Arrb2 / and wild-type (WT) mice 48hours after the second challenge with DNFB (original magnification 200, bar¼ 100 mm). (b) Mean numbers of CD3 þ cells per high-power field (HPF) (±SEM, n ¼ 5 per group). (c) Mean mRNA expression levels of Ifng and Tnfa relative to ubiquitin determined by quantitative real-time reverse-transcriptase–PCR (2 DCT ±SEM, n ¼ 5 per group). (d) Experimental protocol for adoptive lymphocyte (LC) transfer experiments. (e) Mean ear swelling 48hours after the second challenge with DNFB (±SEM, n ¼ 5 mice per group). Experiments were repeated two times with similar results. **Po0.01; ***Po0.001.

Journal: The Journal of investigative dermatology

Article Title: β-arrestin 2 inhibits proinflammatory chemokine production and attenuates contact allergic inflammation in the skin.

doi: 10.1038/jid.2014.117

Figure Lengend Snippet: Figure 3. b-Arrestin 2 deficiency affects the challenge phase of contact hypersensitivity responses. (a) Representative CD3-stained sections of inflamed ear tissue harvested from Arrb2 / and wild-type (WT) mice 48hours after the second challenge with DNFB (original magnification 200, bar¼ 100 mm). (b) Mean numbers of CD3 þ cells per high-power field (HPF) (±SEM, n ¼ 5 per group). (c) Mean mRNA expression levels of Ifng and Tnfa relative to ubiquitin determined by quantitative real-time reverse-transcriptase–PCR (2 DCT ±SEM, n ¼ 5 per group). (d) Experimental protocol for adoptive lymphocyte (LC) transfer experiments. (e) Mean ear swelling 48hours after the second challenge with DNFB (±SEM, n ¼ 5 mice per group). Experiments were repeated two times with similar results. **Po0.01; ***Po0.001.

Article Snippet: For immunohistochemistry, primary antibodies for Gr1 (RB6-8C5, BD Pharmingen, Heidelberg, Germany) or CD3 (Serotec MCA1477, 1:500, antigen unmasking: 10 minutes boiling in pH 6 buffer) were used.

Techniques: Staining, Expressing, Ubiquitin Proteomics, Reverse Transcription

Male breast cancer (MBC) has a full range of expression patterns for eIF4E, 4E-BP1, 4E-BP2 and p4E-BP1. Tissue microarrays containing multiple tumour cores from 337 MBCs were stained as indicated using immunohistochemistry. Cytoplasmic and nuclear expressions in tumour cells were assessed as 0 (negative) or 2–7 (positive, increasing intensity/proportion of positive cells). Representative positive staining is shown at the top of the panel for each antigen. Images shown were scored for cytoplasmic (c) and nuclear (n) expression as follows: eIF4E – c 7, n 6; 4E-BP1 – c 4, n 5; 4E-BP2 – c 5, n 0; p4E-BP1 – c 6, n 3. Frequency distributions of cytoplasmic (black) or nuclear (grey) expression across the cohort are shown below. Mean scores for each case were determined and are represented rounded to the nearest whole number.

Journal: British Journal of Cancer

Article Title: Expression of phosphorylated eIF4E-binding protein 1, but not of eIF4E itself, predicts survival in male breast cancer

doi: 10.1038/bjc.2016.178

Figure Lengend Snippet: Male breast cancer (MBC) has a full range of expression patterns for eIF4E, 4E-BP1, 4E-BP2 and p4E-BP1. Tissue microarrays containing multiple tumour cores from 337 MBCs were stained as indicated using immunohistochemistry. Cytoplasmic and nuclear expressions in tumour cells were assessed as 0 (negative) or 2–7 (positive, increasing intensity/proportion of positive cells). Representative positive staining is shown at the top of the panel for each antigen. Images shown were scored for cytoplasmic (c) and nuclear (n) expression as follows: eIF4E – c 7, n 6; 4E-BP1 – c 4, n 5; 4E-BP2 – c 5, n 0; p4E-BP1 – c 6, n 3. Frequency distributions of cytoplasmic (black) or nuclear (grey) expression across the cohort are shown below. Mean scores for each case were determined and are represented rounded to the nearest whole number.

Article Snippet: Antibodies, dilutions and antigen retrieval: eIF4E (moue monoclonal sc9976; Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1 : 100; boiled for 2 min in a pressure cooker in antigen unmasking solution, Vector, Burlingame, CA, USA); 4E-BP1 (rabbit polyclonal 9452; Cell Signalling Technology (Danvers, MA, USA); 1 : 100; no antigen retrieval); 4E-BP2 (rabbit polyclonal 2845; Cell Signalling Technology); 1 : 100; 12 min full power microwave in pH6 citrate buffer); p4E-BP1 Thr37/46 (rabbit polyclonal 2855; Cell Signalling Technology; 1 : 25; 12 min full power microwave in pH6 citrate buffer).

Techniques: Expressing, Staining, Immunohistochemistry