boiling antigen unmasking solution Search Results


antigen unmasking 189 solution  (Vector Laboratories)
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Antigen Unmasking 189 Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antigen unmasking solution  (Vector Laboratories)
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Vector Laboratories antigen unmasking solution
Antigen Unmasking Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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citrate buffer  (Vector Laboratories)
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unmasking solution  (Agilent technologies)
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Agilent technologies unmasking solution
Unmasking Solution, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3  (Bio-Rad)
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Bio-Rad cd3
Figure 3. b-Arrestin 2 deficiency affects the challenge phase of contact hypersensitivity responses. (a) Representative <t>CD3-stained</t> sections of inflamed ear tissue harvested from Arrb2 / and wild-type (WT) mice 48hours after the second challenge with DNFB (original magnification 200, bar¼ 100 mm). (b) Mean numbers of <t>CD3</t> þ cells per high-power field (HPF) (±SEM, n ¼ 5 per group). (c) Mean mRNA expression levels of Ifng and Tnfa relative to ubiquitin determined by quantitative real-time reverse-transcriptase–PCR (2 DCT ±SEM, n ¼ 5 per group). (d) Experimental protocol for adoptive lymphocyte (LC) transfer experiments. (e) Mean ear swelling 48hours after the second challenge with DNFB (±SEM, n ¼ 5 mice per group). Experiments were repeated two times with similar results. **Po0.01; ***Po0.001.
Cd3, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antigen unmasking buffer  (Vector Laboratories)
86
Vector Laboratories antigen unmasking buffer
Figure 3. b-Arrestin 2 deficiency affects the challenge phase of contact hypersensitivity responses. (a) Representative <t>CD3-stained</t> sections of inflamed ear tissue harvested from Arrb2 / and wild-type (WT) mice 48hours after the second challenge with DNFB (original magnification 200, bar¼ 100 mm). (b) Mean numbers of <t>CD3</t> þ cells per high-power field (HPF) (±SEM, n ¼ 5 per group). (c) Mean mRNA expression levels of Ifng and Tnfa relative to ubiquitin determined by quantitative real-time reverse-transcriptase–PCR (2 DCT ±SEM, n ¼ 5 per group). (d) Experimental protocol for adoptive lymphocyte (LC) transfer experiments. (e) Mean ear swelling 48hours after the second challenge with DNFB (±SEM, n ¼ 5 mice per group). Experiments were repeated two times with similar results. **Po0.01; ***Po0.001.
Antigen Unmasking Buffer, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antigen unmasking solution  (Agilent technologies)
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Agilent technologies antigen unmasking solution
Figure 3. b-Arrestin 2 deficiency affects the challenge phase of contact hypersensitivity responses. (a) Representative <t>CD3-stained</t> sections of inflamed ear tissue harvested from Arrb2 / and wild-type (WT) mice 48hours after the second challenge with DNFB (original magnification 200, bar¼ 100 mm). (b) Mean numbers of <t>CD3</t> þ cells per high-power field (HPF) (±SEM, n ¼ 5 per group). (c) Mean mRNA expression levels of Ifng and Tnfa relative to ubiquitin determined by quantitative real-time reverse-transcriptase–PCR (2 DCT ±SEM, n ¼ 5 per group). (d) Experimental protocol for adoptive lymphocyte (LC) transfer experiments. (e) Mean ear swelling 48hours after the second challenge with DNFB (±SEM, n ¼ 5 mice per group). Experiments were repeated two times with similar results. **Po0.01; ***Po0.001.
Antigen Unmasking Solution, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris-based antigen unmasking solution  (Biozol Diagnostica Vertrieb GmbH)
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Biozol Diagnostica Vertrieb GmbH tris-based antigen unmasking solution
Figure 3. b-Arrestin 2 deficiency affects the challenge phase of contact hypersensitivity responses. (a) Representative <t>CD3-stained</t> sections of inflamed ear tissue harvested from Arrb2 / and wild-type (WT) mice 48hours after the second challenge with DNFB (original magnification 200, bar¼ 100 mm). (b) Mean numbers of <t>CD3</t> þ cells per high-power field (HPF) (±SEM, n ¼ 5 per group). (c) Mean mRNA expression levels of Ifng and Tnfa relative to ubiquitin determined by quantitative real-time reverse-transcriptase–PCR (2 DCT ±SEM, n ¼ 5 per group). (d) Experimental protocol for adoptive lymphocyte (LC) transfer experiments. (e) Mean ear swelling 48hours after the second challenge with DNFB (±SEM, n ¼ 5 mice per group). Experiments were repeated two times with similar results. **Po0.01; ***Po0.001.
Tris Based Antigen Unmasking Solution, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3. b-Arrestin 2 deficiency affects the challenge phase of contact hypersensitivity responses. (a) Representative CD3-stained sections of inflamed ear tissue harvested from Arrb2 / and wild-type (WT) mice 48hours after the second challenge with DNFB (original magnification 200, bar¼ 100 mm). (b) Mean numbers of CD3 þ cells per high-power field (HPF) (±SEM, n ¼ 5 per group). (c) Mean mRNA expression levels of Ifng and Tnfa relative to ubiquitin determined by quantitative real-time reverse-transcriptase–PCR (2 DCT ±SEM, n ¼ 5 per group). (d) Experimental protocol for adoptive lymphocyte (LC) transfer experiments. (e) Mean ear swelling 48hours after the second challenge with DNFB (±SEM, n ¼ 5 mice per group). Experiments were repeated two times with similar results. **Po0.01; ***Po0.001.

Journal: The Journal of investigative dermatology

Article Title: β-arrestin 2 inhibits proinflammatory chemokine production and attenuates contact allergic inflammation in the skin.

doi: 10.1038/jid.2014.117

Figure Lengend Snippet: Figure 3. b-Arrestin 2 deficiency affects the challenge phase of contact hypersensitivity responses. (a) Representative CD3-stained sections of inflamed ear tissue harvested from Arrb2 / and wild-type (WT) mice 48hours after the second challenge with DNFB (original magnification 200, bar¼ 100 mm). (b) Mean numbers of CD3 þ cells per high-power field (HPF) (±SEM, n ¼ 5 per group). (c) Mean mRNA expression levels of Ifng and Tnfa relative to ubiquitin determined by quantitative real-time reverse-transcriptase–PCR (2 DCT ±SEM, n ¼ 5 per group). (d) Experimental protocol for adoptive lymphocyte (LC) transfer experiments. (e) Mean ear swelling 48hours after the second challenge with DNFB (±SEM, n ¼ 5 mice per group). Experiments were repeated two times with similar results. **Po0.01; ***Po0.001.

Article Snippet: For immunohistochemistry, primary antibodies for Gr1 (RB6-8C5, BD Pharmingen, Heidelberg, Germany) or CD3 (Serotec MCA1477, 1:500, antigen unmasking: 10 minutes boiling in pH 6 buffer) were used.

Techniques: Staining, Expressing, Ubiquitin Proteomics, Reverse Transcription